Abstract- Bacterial infusion in concrete is a method of repairing surface cracks in concrete by introducing bacteria into the concrete matrix prior to pouring it into the mold. Microbial concrete’s crack and corrosion resistance makes it highly appealing, leading to enhanced strength and greater interest. Bacterial activity and their resulting precipitates modify the microstructure of concrete, hence enhancing its impermeability. This study examined the durability and fracture healing characteristics of concrete infused with bacillus subtilis bacteria. Bacterial impregnated concrete exhibited superior durability in comparison to conventional M25 concrete. Submerging bacterial concrete in water resulted in the reduction in crack breadth, demonstrating its inherent ability to self-heal